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Title   원발성 위암에서 c-erbB-2 종양 유전자의 증폭에 대한 연구 ( Amplification of the c-erbB-2 Prto - Oncogene in Primary Human Gastric Cancers )
Publicationinfo   1995 Jan; 027(02): 195-202.
Key_word   Gastric cancer, c-erbB-2 Amplification
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Abstract   The c-er6B-2 proto-oncogene has been found to be amplified in many human tumors induding breast, stomach, kidney and lung cancers. In order to evaluate the amplification status of c-erbB-2 proto-oncogene in stomach cancer which is the most common cancer in Korea, fortyfive sets of specimens including tumor tissue and normal stomach tissue(5 cm at least away from primary tumor, histologically normal)were measured by Southern hybridization technique. Ten micrograms of EcoRI-digested DNA was electrophoresed in an 0.8% agarose gel and transferred to a nylon memebrane and hybridized with a c-erbB-2 probe, washed under stringent conditions and autoradiographed against Hyperfilm with intenaifying screens at 70`C, for 3 days. In the results. two of the 45 gastric carcinomas(4.4%) were amplified 2-to-4-fold. Thus, the amplified c-erbB-2 gene may have a role in the evolution of gastric carcinoma, as dose in some carcinoma.
저 자   최철원(Chul Won Choi),서재홍(Jae Hong Seo),김병수(Byung Soo Kim),신상원(Sang Won Shin),김준석(Jun Suk Kim),목영재(Young Jae Mok),김종석(Jong Suk Kim),구범환(Bum Hwan Koo)